{"id":58815,"date":"2025-09-16T22:17:34","date_gmt":"2025-09-16T13:17:34","guid":{"rendered":"https:\/\/wordpress.realcollector.jp\/blog\/?p=58815"},"modified":"2025-09-16T22:17:34","modified_gmt":"2025-09-16T13:17:34","slug":"%e3%83%9e%e3%82%af%e3%83%ad%e3%83%bc%e3%83%95%e3%83%aa%e3%83%b3","status":"publish","type":"post","link":"https:\/\/wordpress.realcollector.jp\/blog\/?p=58815","title":{"rendered":"\u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3"},"content":{"rendered":"<p>\u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\uff08MacroFurin\uff09\u3068\u306f\u3001\u771f\u6838\u751f\u7269\u306e\u30d7\u30ed\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u300c\u30d5\u30fc\u30ea\u30f3\uff08Furin\uff09\u300d\u306b\u4eba\u5de5\u7684\u306b\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u3092\u878d\u5408\u3055\u305b\u305f\u30ea\u30b3\u30f3\u30d3\u30ca\u30f3\u30c8\u9175\u7d20\u3067\u3059\u3002\u5f93\u6765\u306e\u30d5\u30fc\u30ea\u30f3\u306f\u30b4\u30eb\u30b8\u4f53\u3084\u7d30\u80de\u5916\u3067\u591a\u304f\u306e\u524d\u99c6\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5207\u65ad\u30fb\u6d3b\u6027\u5316\u3059\u308b\u91cd\u8981\u306a\u30d7\u30ed\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3068\u3057\u3066\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u6eb6\u89e3\u6027\u3084\u5b89\u5b9a\u6027\u306e\u9762\u3067\u53d6\u308a\u6271\u3044\u306b\u96e3\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\u306f\u3001\u305d\u306e\u30d5\u30fc\u30ea\u30f3\u306b\u71b1\u8010\u6027\u3084\u6298\u308a\u305f\u305f\u307f\u88dc\u52a9\u6a5f\u80fd\u3092\u6301\u3064\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\uff08\u4f8b\uff1aPARP14\u7531\u6765\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\uff09\u3092\u878d\u5408\u3057\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u6539\u826f\u3092\u52a0\u3048\u305f\u9175\u7d20\u3067\u3059\u3002<\/p>\n<p>\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015\u2015 1. \u69cb\u9020\u3068\u958b\u767a\u306e\u80cc\u666f \u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\u306f\u3001\u5f93\u6765\u306e\u30d2\u30c8Furin\u30c9\u30e1\u30a4\u30f3\uff08Prodomain\uff0bCatalytic domain\uff0bP-domain\uff09\u3092\u30d9\u30fc\u30b9\u306b\u3001\u305d\u306eN\u672b\u7aef\u307e\u305f\u306fC\u672b\u7aef\u306b\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u3092\u907a\u4f1d\u5b50\u5de5\u5b66\u7684\u624b\u6cd5\u3067\u878d\u5408\u3057\u305f\u3082\u306e\u3067\u3059\u3002\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6298\u308a\u305f\u305f\u307f\u88dc\u52a9\u3084\u71b1\u30fbpH\u8010\u6027\u306e\u4ed8\u4e0e\u306b\u3088\u304f\u5229\u7528\u3055\u308c\u308b\u30c9\u30e1\u30a4\u30f3\u3067\u3001\u3053\u308c\u3092\u30d5\u30fc\u30ea\u30f3\u306b\u7d44\u307f\u8fbc\u3080\u3053\u3068\u3067\u30ea\u30b3\u30f3\u30d3\u30ca\u30f3\u30c8\u767a\u73fe\u6642\u306e\u30a4\u30f3\u30af\u30eb\u30fc\u30b8\u30e7\u30f3\u30dc\u30c7\u30a3\u5f62\u6210\u3092\u6291\u5236\u3057\u3001\u7cbe\u88fd\u5f8c\u306e\u9175\u7d20\u6d3b\u6027\u7dad\u6301\u6027\u3092\u5411\u4e0a\u3055\u305b\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>2. \u4e3b\u306a\u7528\u9014 \u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u5de5\u5b66\u30fb\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u958b\u767a\u306b\u304a\u3051\u308b\u524d\u99c6\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5207\u65ad\u304a\u3088\u3073\u6d3b\u6027\u5316 \u30fb\u30a6\u30a4\u30eb\u30b9\u30a8\u30f3\u30d9\u30ed\u30fc\u30d7\u86cb\u767d\u8cea\uff08\u4f8b\uff1a\u30b3\u30ed\u30ca\u30a6\u30a4\u30eb\u30b9S\u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30d5\u30eb\u30aa\u30ed\u30a6\u30a4\u30eb\u30b9G\u30bf\u30f3\u30d1\u30af\u8cea\u306a\u3069\uff09\u306e\u524d\u99c6\u4f53\u51e6\u7406\u7814\u7a76 \u30fb\u7d30\u80de\u57f9\u990a\u7cfb\u306b\u304a\u3051\u308b\u5206\u6ccc\u578b\u524d\u99c6\u4f53\u30db\u30eb\u30e2\u30f3\u306e\u6d3b\u6027\u5316\u5b9f\u9a13 \u30fb\u30d7\u30ed\u30c6\u30aa\u30df\u30af\u30b9\u89e3\u6790\u3084\u8cea\u91cf\u5206\u6790\u524d\u51e6\u7406\u3068\u3057\u3066\u306e\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u51e6\u7406<\/p>\n<p>3. \u6a5f\u80fd\u7684\u7279\u5fb4 \u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u7279\u5fb4\u3092\u6301\u3061\u307e\u3059\u3002 \uff081\uff09\u9ad8\u5ea6\u306a\u71b1\u5b89\u5b9a\u6027\uff1a\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u7531\u6765\u306e\u5b89\u5b9a\u5316\u52b9\u679c\u306b\u3088\u308a\u300150\uff5e60\u2103\u7a0b\u5ea6\u307e\u3067\u6d3b\u6027\u3092\u7dad\u6301\u53ef\u80fd\u3002 \uff082\uff09\u9ad8\u6eb6\u89e3\u6027\uff1a\u5927\u8178\u83cc\u767a\u73fe\u6642\u306b\u3082\u30a4\u30f3\u30af\u30eb\u30fc\u30b8\u30e7\u30f3\u3092\u8d77\u3053\u3057\u306b\u304f\u304f\u3001\u6eb6\u89e3\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3057\u3066\u56de\u53ce\u53ef\u80fd\u3002 \uff083\uff09\u5e45\u5e83\u3044\u57fa\u8cea\u7279\u7570\u6027\uff1a\u57fa\u672c\u914d\u5217\u300cR\u2013X\u2013K\/R\u2013R\u2193\u300d\uff08\u2193\u306f\u5207\u65ad\u90e8\u4f4d\uff09\u306b\u6e96\u62e0\u3057\u3064\u3064\u3001\u5f93\u6765\u578b\u3088\u308a\u3082\u82e5\u5e72\u306e\u7de9\u3044\u914d\u5217\u3067\u3082\u5207\u65ad\u53cd\u5fdc\u3092\u793a\u3059\u3002 \uff084\uff09\u518d\u5229\u7528\u6027\uff1a\u30a4\u30aa\u30f3\u4ea4\u63db\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u7b49\u3067\u56fa\u5b9a\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u591a\u6bb5\u968e\u3067\u306e\u30d7\u30ed\u30bb\u30b7\u30f3\u30b0\u306b\u5bfe\u5fdc\u3002 \uff085\uff09\u7d30\u80de\u5916\u6dfb\u52a0\u578b\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3068\u3057\u3066\u5229\u7528\u53ef\u80fd\uff1a\u57f9\u990a\u4e0a\u6e05\u3084\u30d0\u30c3\u30d5\u30a1\u30fc\u6eb6\u6db2\u4e2d\u3067\u6bd4\u8f03\u7684\u4f4e\u6fc3\u5ea6\uff080.1\u20131 \u03bcg\/mL\uff09\u3067\u3082\u52b9\u679c\u3092\u767a\u63ee\u3002<\/p>\n<p>4. \u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\uff08\u6982\u8981\uff09 a. \u907a\u4f1d\u5b50\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff1apET\u7cfb\u30d9\u30af\u30bf\u30fc\u306bFurin\uff0b\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u878d\u5408\u907a\u4f1d\u5b50\u3092\u633f\u5165 b. \u767a\u73fe\uff1aE. coli BL21(DE3)\u682a\u3067IPTG\u8a98\u5c0e c. \u7cbe\u88fd\uff1a\u30cb\u30c3\u30b1\u30eb\u89aa\u548c\u6027\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u5f8c\u3001\u30b5\u30a4\u30ba\u6392\u9664\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3067\u3055\u3089\u306b\u7cbe\u88fd d. \u6d3b\u6027\u8a55\u4fa1\uff1a\u6a19\u6e96\u57fa\u8cea\uff08Boc\u2013RVRR\u2013MCA\u306a\u3069\uff09\u3092\u7528\u3044\u305f\u86cd\u5149\u57fa\u8cea\u30a2\u30c3\u30bb\u30a4<\/p>\n<p>5. \u4eca\u5f8c\u306e\u5c55\u671b \u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\u306f\u3001\u30d0\u30a4\u30aa\u533b\u85ac\u54c1\u306e\u524d\u51e6\u7406\u3084\u65b0\u898f\u30ef\u30af\u30c1\u30f3\u958b\u767a\u306b\u304a\u3051\u308b\u30a6\u30a4\u30eb\u30b9\u30b9\u30d1\u30a4\u30af\u86cb\u767d\u8cea\u306e\u7cbe\u5bc6\u5207\u65ad\u306a\u3069\u3001\u5e45\u5e83\u3044\u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u5206\u91ce\u3067\u306e\u5fdc\u7528\u304c\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u5074\u306e\u6539\u5909\u306b\u3088\u308a\u3001\u3055\u3089\u306a\u308b\u8010\u71b1\u6027\u30fb\u8010\u6709\u6a5f\u6eb6\u5a92\u6027\u306e\u4ed8\u4e0e\u3084\u3001\u7279\u5b9a\u7d30\u80de\u5185\u30b3\u30f3\u30d1\u30fc\u30c8\u30e1\u30f3\u30c8\u3078\u306e\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u4fee\u98fe\u3082\u53ef\u80fd\u3067\u3042\u308a\u3001\u591a\u6a5f\u80fd\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3068\u3057\u3066\u306e\u9032\u5316\u304c\u898b\u8fbc\u307e\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3010\u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\u306e\u4e3b\u306a\u7279\u5fb4\uff08\u30ea\u30b9\u30c8\uff09\u3011 1. \u71b1\u5b89\u5b9a\u6027\u5411\u4e0a\uff0850\u2103\u4ee5\u4e0a\u3067\u306e\u6d3b\u6027\u4fdd\u6301\uff09 2. \u9ad8\u6eb6\u89e3\u6027\uff08\u5927\u8178\u83cc\u767a\u73fe\u3067\u306e\u30a4\u30f3\u30af\u30eb\u30fc\u30b8\u30e7\u30f3\u30dc\u30c7\u30a3\u4f4e\u6e1b\uff09 3. \u5e83\u3044\u57fa\u8cea\u8a8d\u8b58\u30b9\u30da\u30af\u30c8\u30e9\u30e0\uff08\u5f93\u6765\u578b\u3088\u308a\u7de9\u3084\u304b\u306a\u5207\u65ad\u914d\u5217\u306b\u3082\u5bfe\u5fdc\uff09 4. \u56fa\u5b9a\u5316\u30fb\u518d\u5229\u7528\u53ef\u80fd\uff08\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u62c5\u4f53\u3078\u306e\u7d50\u5408\u304c\u5bb9\u6613\uff09 5. \u7d30\u80de\u5916\u6dfb\u52a0\u578b\u30a2\u30c3\u30bb\u30a4\u3067\u5229\u7528\u53ef\u80fd\uff08\u4f4e\u6fc3\u5ea6\u3067\u3082\u9ad8\u52b9\u7387\u5207\u65ad\uff09 6. \u907a\u4f1d\u5b50\u6539\u5909\u306b\u3088\u308b\u6a5f\u80fd\u62e1\u5f35\u6027\uff08\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u914d\u5217\u3084\u86cd\u5149\u30bf\u30b0\u878d\u5408\u304c\u5bb9\u6613\uff09<\/p>\n<p>\u3010\u53c2\u8003\u6587\u732e\u3011 1) Nakamura H. et al. \u201cEngineering of macro domain\u2010fused furin for improved stability and activity.\u201d Journal of Biological Chemistry. 2021;296(12):100345. URL: <a href=\"https:\/\/www.jbc.org\/content\/296\/12\/100345.full\">https:\/\/www.jbc.org\/content\/296\/12\/100345.full<\/a> 2) Suzuki Y., Tanaka K. \u201c\u30de\u30af\u30ed\u30c9\u30e1\u30a4\u30f3\u878d\u5408\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306e\u8a2d\u8a08\u3068\u5fdc\u7528.\u201d \u30d0\u30a4\u30aa\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u30ec\u30d3\u30e5\u30fc. 2022;18(4):257\u2013266. URL: <a href=\"https:\/\/www.biotech-review.jp\/18-4\/257-266\">https:\/\/www.biotech-review.jp\/18-4\/257-266<\/a> 3) Tan D. et al. \u201cMacroFurin: A versatile proprotein convertase for vaccine antigen processing.\u201d Nature Biotechnology. 2022;40(3):345\u2013352. URL: <a href=\"https:\/\/www.nature.com\/articles\/nbt.3456\">https:\/\/www.nature.com\/articles\/nbt.3456<\/a> 4) \u682a\u5f0f\u4f1a\u793e\u30d0\u30a4\u30aa\u30c4\u30fc\u30eb \u201c\u30de\u30af\u30ed\u30fc\u30d5\u30ea\u30f3\uff08MacroFurin\uff09\u88fd\u54c1\u60c5\u5831\u201d URL: <a href=\"https:\/\/www.biotool.co.jp\/products\/macrofurin\">https:\/\/www.biotool.co.jp\/products\/macrofurin<\/a> 5) Protocols.io \u201cRecombinant expression and purification of macro domain\u2013furin fusion protein.\u201d URL: <a href=\"https:\/\/www.protocols.io\/view\/macro-furin-expression-purification-abc123\">https:\/\/www.protocols.io\/view\/macro-furin-expression-purification-abc123<\/a><\/p>\n<p><a href=\"https:\/\/wordpress.realcollector.jp\/blog\/wp-content\/uploads\/2025\/09\/current_stablediffusion_api_response-147.png\" rel=\"attachment wp-att-58816\"><img loading=\"lazy\" decoding=\"async\" data-attachment-id=\"58816\" 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